The method of claim 9, wherein the cell comprises a eukaryotic cell.ġ2. The method of claim 9, wherein the cell comprises a prokaryotic cell.ġ1. The method of claim 1, wherein the target locus of interest is comprised in a DNA within a cell.ġ0. The method of claim 1, wherein the target locus of interest is comprised in a DNA molecule in vitro.ĩ. The method of claim 1, wherein the effector protein comprises C2c3p.Ĩ. The method of claim 1, wherein the effector protein is encoded by a subtype V-C CRISPR-Cas loci.ħ. The method of claim 1, wherein the effector protein comprises C2c1p.Ħ. The method of claim 1, wherein the effector protein is encoded by a subtype V-B CRISPR-Cas loci.ĥ. The method of claim 1, wherein the modification of the target locus of interest comprises a strand break.Ĥ.
The method of claim 1, wherein the target locus of interest comprises DNA.ģ. A method of modifying a target locus of interest, the method comprising delivering to said locus a non-naturally occurring or engineered composition comprising a Type V CRISPR-Cas loci effector protein and one or more nucleic acid components, wherein at least the one or more nucleic acid components is engineered and the effector protein forms a complex with the one or more nucleic acid components and upon binding of the said complex to the target locus of interest the effector protein induces a modification of the target locus of interest, wherein the Type V CRISPR-Cas loci effector protein comprises C2c1p or C2c3p.Ģ.
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